DNA

Part:BBa_K2100039

Designed by: Wangui Mbuguiro   Group: iGEM16_MIT   (2016-10-17)


pEXPR pBM3R1 - eYFP

This construct is the result of a gateway combining a BM3R1 repressible promoter upstream of the eYFP gene, a yellow fluorescent protein. pBM3R1 consists of one repressible site upstream and four repressible sites downstream of the minimal CMV promoter. A VP-16 Gal4 protein complex recruits the transcription machinery of the promoter. The BM3R1 repressor inhibits the activator and transcription machinery from transcribing the eYFP gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 242
    Illegal PstI site found at 481
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 242
    Illegal NheI site found at 115
    Illegal PstI site found at 481
    Illegal NotI site found at 4
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 242
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 242
    Illegal PstI site found at 481
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 242
    Illegal PstI site found at 481
    Illegal AgeI site found at 264
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 108
    Illegal BsaI site found at 235


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